کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6297743 | 1617778 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
3,4-Dimethylpyrazole phosphate (DMPP) reduces activity of ammonia oxidizers without adverse effects on non-target soil microorganisms and functions
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) is widely used within agriculture to reduce nitrate leaching and improve nitrogen use efficiency of fertilizers, but few studies examined effects on non-target soil functions and microorganisms, i.e. other than the intended delay of ammonia oxidation. We investigated effects of DMPP amendment equivalent to 0 (Control), 1 (regular dose, RD) or 10 (high dose, HD) kg haâ1 in a sandy loam grassland soil at 50% water-filled pore space. Following incubation for 1, 7 or 14 d, soil was analyzed for fluorescein diacetate hydrolysis, dehydrogenase activity, phospholipid fatty acid composition and potential ammonia oxidation. DMPP showed no significant non-target effects (p > 0.05), but a possible stress response in HD was indicated by a factor analysis of phospholipid fatty acid composition. There was a strong DMPP inhibition on potential ammonia oxidation which was still significant (p < 0.05) in HD after 14 d. In separate treatments receiving 50 mg NH4+-N kgâ1 dry soil in addition to DMPP, the inhibition of nitrate accumulation was similar in RD and HD at around 75%. Abundances of the gene amoA from ammonia oxidizing bacteria (AOB) and archaea (AOA) were quantified, and cell-specific nitrification rates were estimated. There was a general trend of increasing AOA and AOB abundance towards the end of incubation irrespective of DMPP treatment, whereas cell-specific activity of AOA and/or AOB was reduced in the presence of DMPP. Overall, this study indicated that DMPP effectively inhibited nitrification activity without effects on ammonia oxidizer populations, as well as non-target soil microorganisms or functions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Soil Ecology - Volume 105, September 2016, Pages 67-75
Journal: Applied Soil Ecology - Volume 105, September 2016, Pages 67-75
نویسندگان
Xianwang Kong, Yunfeng Duan, Andreas Schramm, Jørgen Eriksen, Søren O. Petersen,