Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina

► Selective count and PCR-RFLP assays set up to study pseudomonads in agricultural soils. ► Novel primers were designed to set up PCR-RFLP targeting gacA and oprF genus specific markers. ► Specificity of plating and molecular detection was verified by sequencing. ► Results highlight the taxonomic power of oprF gene. ► Pseudomonads CFUs and groups varied among sites, treatments, and rhizosphere vs bulk soil.