کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6301282 | 1618008 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bait and scrape: An approach for assessing biofilm microbial communities on organic media used for gas-phase biofiltration
ترجمه فارسی عنوان
طعمه و خرچنگ: یک رویکرد برای ارزیابی جوامع میکروبی بیوفیلم بر روی مواد آلی که برای بیوفیلتراسیون فاز گاز استفاده می شود
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کلمات کلیدی
بیوفیلم، بیوفیلتر، بیوماس میکروبی، قارچ، بیومارکر، چوب،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
چکیده انگلیسی
Gas-phase biofilters offer effective pollution control for agricultural effluents, but a better understanding of microbial communities responsible for capture and degradation is needed to improve process control. In this study, we developed a wood bait and optimized microbial biofilm sampling for monitoring microbial biomarkers (microbial C, ergosterol, DNA) in a full-scale biofilter. Results demonstrated that targeting biofilm dynamics required removing the biofilm from the wood substrates prior to biomarker extraction. We identified a sampling threshold for these biofilms of â¥100 mg for accurate and low variability biomarker measurement, a threshold that can inform analyses in other systems or using other approaches (i.e. DNA sequencing). Using this approach in a full-scale biofilter revealed that the fungal contribution (as ergosterol) to total microbial biomass was greatest in the most desiccation-prone area of the biofilter. This observation is in-line with results from previous lab-scale studies and could be due, in part, to connectivity between fungal hyphae growing in biofilms and the wood baits, shown by confocal microscopy. This work provides a targeted sampling strategy for microbial biofilms in gas-phase biofiltration, adaptable to other pollution control bioreactors and that can be used to study microbial community dynamics in full-scale systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecological Engineering - Volume 91, June 2016, Pages 50-57
Journal: Ecological Engineering - Volume 91, June 2016, Pages 50-57
نویسندگان
Jason P. Oliver, Kevin A. Janni, Jonathan S. Schilling,