Ranking Gene Ontology terms for predicting non-classical secretory proteins in eukaryotes and prokaryotes

► Use Gene Ontology (GO) terms as the only one type of input features. ► Identify two small sets of 436 and 158 GO terms for eukaryotes and prokaryotes. ► The Sec-GO method performs better (96.7%) than SPRED (82.2%) in eukaryotes. ► The Sec-GO method performs better (94.5%) than NClassG+ (90.0%) in prokaryotes.