کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6382155 | 1625938 | 2015 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of Ahr signaling by Nrf2 during development: Effects of Nrf2a deficiency on PCB126 embryotoxicity in zebrafish (Danio rerio)
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کلمات کلیدی
CYP1ADioxin-like compoundtBOOHPCB126DLChpfNrf2DPFAHRAHRRTBHQPAH3,3′,4,4′,5-pentachlorobiphenyl - 3،3 '، 4،4'، 5-پنتاکلروبوفنیلkeap1 - buy1DMSO - DMSOROS - ROSdays post fertilization - بارور شدن روزانهDimethyl sulfoxide - دیمتیل سولفواکسیدhours post fertilization - ساعت پس از لقاحantioxidant response element - عنصر پاسخ آنتی اکسیدانARE - هستندPolycyclic aromatic hydrocarbon - هیدروکربن آروماتیک چند حلقه ایKelch-like ECH-associated protein 1 - پروتئین مرتبط با ECH کلچ 1Reactive oxygen species - گونههای فعال اکسیژنaryl hydrocarbon receptor - گیرنده آرویل هیدروکربنaryl hydrocarbon receptor repressor - گیرنده گیرنده آرویل هیدروکربن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Regulation of Ahr signaling by Nrf2 during development: Effects of Nrf2a deficiency on PCB126 embryotoxicity in zebrafish (Danio rerio) Regulation of Ahr signaling by Nrf2 during development: Effects of Nrf2a deficiency on PCB126 embryotoxicity in zebrafish (Danio rerio)](/preview/png/6382155.png)
چکیده انگلیسی
The embryotoxicity of co-planar PCBs is regulated by the aryl hydrocarbon receptor (Ahr), and has been reported to involve oxidative stress. Ahr participates in crosstalk with another transcription factor, Nfe2l2, or Nrf2. Nrf2 binds to antioxidant response elements to regulate the adaptive response to oxidative stress. To explore aspects of the crosstalk between Nrf2 and Ahr and its impact on development, we used zebrafish (Danio rerio) with a mutated DNA binding domain in Nrf2a (nrf2afh318/fh318), rendering these embryos more sensitive to oxidative stress. Embryos were exposed to 2Â nM or 5Â nM PCB126 at 24Â h post fertilization (prim-5 stage of pharyngula) and examined for gene expression and morphology at 4 days post fertilization (dpf; protruding - mouth stage). Nrf2a mutant eleutheroembryos were more sensitive to PCB126 toxicity at 4Â dpf, and in the absence of treatment also displayed some subtle developmental differences from wildtype embryos, including delayed inflation of the swim bladder and smaller yolk sacs. We used qPCR to measure changes in expression of the nrf gene family, keap1a, keap1b, the ahr gene family, and known target genes. cyp1a induction by PCB126 was enhanced in the Nrf2a mutants (156-fold in wildtypes vs. 228-fold in mutants exposed to 5Â nM). Decreased expression of heme oxygenase (decycling) 1 (hmox1) in the Nrf2a mutants was accompanied by increased nrf2b expression. Target genes of Nrf2a and AhR2, NAD(P)H:quinone oxidoreductase 1 (nqo1) and glutathione S-transferase, alpha-like (gsta1), showed a 2-5-fold increase in expression in the Nrf2a mutants as compared to wildtype. This study elucidates the interaction between two important transcription factor pathways in the developmental toxicity of co-planar PCBs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 167, October 2015, Pages 157-171
Journal: Aquatic Toxicology - Volume 167, October 2015, Pages 157-171
نویسندگان
Michelle E. Rousseau, Karilyn E. Sant, Linnea R. Borden, Diana G. Franks, Mark E. Hahn, Alicia R. Timme-Laragy,