کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
640502 1456972 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Separation of protein mixtures by an integrated electro-ultrafiltration-electrodialysis process
ترجمه فارسی عنوان
جداسازی مخلوط پروتئین با فرآیند الکترود اولترافیلتراسیون الکترو دی دیالیز
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
چکیده انگلیسی
An integrated process for improving selectivity and permeate flux in ultrafiltration based protein fractionation was developed by combining electrodialysis (ED) with electro-ultrafiltration (EUF). The performance of such a process was first investigated using individual proteins (bovine serum albumin and lysozyme) and then with a mixture of the two. The experimental results showed that as with EUF on its own, the build-up of lysozyme concentration polarization near the cation exchange membrane in the permeate compartment significantly affected ion migration and led to change in pH and conductivity of the feed solution during an EUF-ED process with lysozyme, or bovine serum albumin and lysozyme mixture. As compared with EUF, EUF-ED of protein mixture resulted in a 20% increase in permeate flux with the lysozyme transmission remaining the same. The demineralization that occurred during EUF-ED made this process suitable for protein separation from the feed solutions with high conductivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 147, 16 June 2015, Pages 32-43
نویسندگان
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