کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6406115 1628787 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro proliferation and ex vitro rooting of microshoots of commercially important rabbiteye blueberry (Vaccinium ashei Reade) using spectral lights
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش باغداری
پیش نمایش صفحه اول مقاله
In vitro proliferation and ex vitro rooting of microshoots of commercially important rabbiteye blueberry (Vaccinium ashei Reade) using spectral lights
چکیده انگلیسی


- First report on micropropagation of rabbiteye blueberry using LEDs with ventilation.
- Combining 80% red with 20% blue LEDs improved plant growth in vitro and ex vitro.
- Healthy plants at both stages were produced with ventilation.
- LEDs are recommended to replace fluorescent lamps in blueberry micropropagation.

Efficient protocols for in vitro shoot growth and ex vitro root formation from in vitro-derived shoots under light-emitting diodes (LEDs) with simple ventilation were developed for rabbiteye blueberry cv. 'Titan'. Red LEDs promoted shoot elongation while blue LEDs induced short shoots with enhanced accumulation of leaf chlorophyll contents. A combined treatment of 80% red with 20% blue LEDs was found the most suitable for plant growth, and more effective than 50% red mixed with 50% blue LEDs or fluorescent lamps as the control treatment. The use of ventilated vessels with ambient CO2 enrichment from a growth chamber resulted in healthy plants having greatly improved shoot length, leaf expansion, leaf chlorophyll contents and dry weight of both shoots and roots. In vitro-cultured shoots could be directly rooted at 100% under non-sterile conditions using a perlite-peat mixture with careful controls of high humidity. These results suggest that LEDs, particularly when coupled with ventilated vessels, should be used as a primary light source for replacing conventional fluorescent tubes in large-scale production of rabbiteye blueberries.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 211, 1 November 2016, Pages 248-254
نویسندگان
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