کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6407484 | 1628833 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning and functional characterization of dihydroflavonol-4-reductase gene from Calibrachoa hybrida
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular cloning and functional characterization of dihydroflavonol-4-reductase gene from Calibrachoa hybrida Molecular cloning and functional characterization of dihydroflavonol-4-reductase gene from Calibrachoa hybrida](/preview/png/6407484.png)
چکیده انگلیسی
We constructed 35S::CaDFR overexpression vector and transformed it into three cultivars of Petunia hybrida using the Agrobacterium-mediated method. Multiple transformants were selected in the presence of 50Â mg/l kanamycin antibiotic and further verified by polymerase chain reaction and Southern blotting analyses. We observed that the bud and flower color of transgenic petunia altered comparing with that of control plants. The DFR activities varied in most color-changed transgenic petunias. These results confirm the identity and function of CaDFR and further suggest that integration of the CaDFR can be used to change flower color of transgenic plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 165, 22 January 2014, Pages 398-403
Journal: Scientia Horticulturae - Volume 165, 22 January 2014, Pages 398-403
نویسندگان
Yunxia Chu, Junsong Pan, Aizhong Wu, Run Cai, Hairong Chen,