کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
640860 | 1456981 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Using two-phase solvent systems for sample pretreatment increases yield of counter-current chromatography: Anemarrhena asphodeloides saponins, a case study
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This paper reports a way to increase the production yield of counter-current chromatography by using two-phase solvent systems in sample pretreatment. This strategy succeeds in the case of anti-EV71 saponin preparation from Anemarrhena asphodeloides. Briefly, 15Â g of water extract was refined by solvent partition with ethyl acetate/1-butanol/water (1:4:5, v/v) to yield 4.17Â g of the upper part, which in turns yielded 635Â mg of timosaponin B-II (purity 96.5%) and 672Â mg of anemarsaponin B (97.8%) after gradient CCC separation with 1-butanol/water (containing 0.01Â mol/L CuSO4 in water, 1:1, v/v) and ethyl acetate/1-butanol/water (3:17:20, v/v/v). In a similar way, the ethanol extract (8Â g) was partitioned between the diphase solvent system of ethyl acetate/1-butanol/water (4:1:5) to get 2.16Â g of refined fraction and yielded 358Â mg of timosaponin A-III (96.4%) by CCC elution using ethyl acetate-methanol-water (4:1:5, v/v/v). To remove mangiferin, the salt CuSO4 was added; it formed a complex with mangiferin, thereby enlarging the separation coefficient between mangiferin and timosaponin B-II.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 138, 10 December 2014, Pages 138-143
Journal: Separation and Purification Technology - Volume 138, 10 December 2014, Pages 138-143
نویسندگان
Meng-Shun Liu, Siu-Leung Chau, Dik-Lung Ma, Chung-Hang Leung, Zhao-Xiang Bian, Ai-Ping Lu, Hong-Xi Xu, Quan-Bin Han,