Purification of α-galactosidase from pepino (Solanum muricatum) by three-phase partitioning

► Pepino (Solanum muricatum) was found to be very good source for α-galactosidase. ► Three-phase partitioning (TPP) is used for bioseparation of α-galactosidase. ► TPP protocol is very rapid, simple and highly efficient for pepino α-galactosidase. ► Properties of the enzyme are very attractive for its industrial applications.