Integrated fluidized bed affinity recovery of an anti-MUC1 mucin recombinant diabody from Escherichia coli lysates

The fluidized bed affinity recovery of C595 diabody fragment (dbFv) from Escherichia coli lysates in an integrated system mode has been investigated. An amino acid epitope sequence, RPAP was immobilized onto Upfront adsorbent (ρ = 1.35 g cm−3). The recovery performance of various feedstocks (clarified, unclarified and concentrated unclarified feedstocks) were compared and evaluated. It was demonstrated that the values of operational productivity for clarified and unclarified feedstock were 198.18 and 129.11 mg L−1, respectively. A relatively high elution yield (87–93%) was achieved for all types of feedstock evaluated.

► The fluidized bed recovery of C595 diabody fragment from Escherichia coli lysates has been studied.
► An amino acid epitope sequence was immobilized onto Upfront adsorbent.
► The value of operational productivity for unclarified feedstock was 129.11 mg L−1.