Temperature and magnetic dual responsive microparticles for DNA separation

► Temperature responsive magnetic microparticles have been prepared for DNA separation. ► Electrostatic attraction is the main force for DNA adsorption. ► DNA adsorption is highly affected by pH and significantly affected by temperature. ► DNA adsorption isotherm curve is well fitted with Langmuir model. ► Higher temperature, basic pH and higher salt concentration are favorable for DNA desorption.