Studies of protein partition in non conventional aqueous two-phase systems as method to purify trypsinogen and alpha-chymotrypsinogen from bovine pancreas

► Trypsinogen and chymotrypsinogen partitioning in aqueous biphasic systems was assayed. ► A mixture highly pure (95%) of TRPz and ChTRPz is recovered after one extraction step. ► No clarification stage is required. ► A final back extraction allow to remove polyethyleneglycol drastically.