کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
644081 884413 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification and quantification of ginsenoside Rb3 and Rc from crude extracts of caudexes and leaves of Panax notoginseng
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
Purification and quantification of ginsenoside Rb3 and Rc from crude extracts of caudexes and leaves of Panax notoginseng
چکیده انگلیسی

In this work, the separation of ginsenoside Rb3 and Rc from the crude extracts of caudexes and leaves of Panax notoginseng (CECLPN) was studied with reversed-phase high-performance liquid chromatography (RP-HPLC). The chromatographic separation was achieved using a SynChropak C18 column (at 25 °C). A satisfied separation of the compounds was obtained in less than 40 min with a flow rate of 1.0 ml/min. Chromatography was performed using ternary mobile phase composed of methanol, water and phosphoric acid, 65:33:1.4 (v/v). UV detection was accomplished at 203 nm. Ginsenoside Rb3 and Rc found in the CECLPN constitute up to 38.25% and 26.65% of the dry powder, respectively. Ginsenoside Rb3 and Rc were prepared by semi-preparative HPLC/ELSD using gradient elution system of methanol–water = 70:30 → 65:35 at a flow rate of 30 ml/min with a sample load of 300–400 mg. With this chromatographic condition, each individual ginsenoside Rb3 and Rc in purity of more than 97% were gained. The products were confirmed by spectroscopic (UV, IR, ESI-MS/MS, NMR) and HPLC methods. The methods were validated for linearity, precision, recovery, limits of detection (LOD) and limits of quantification (LOQ). The results show that the proposed method appears to be an adequate method for quality control of CECLPN and a useful tool for pharmaceutical study of ginsenosides.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 54, Issue 2, 1 April 2007, Pages 198–203
نویسندگان
, , , , ,