کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6467498 1423258 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of residual lignins present in cholinium ionic liquid-pretreated rice straw on the enzymatic hydrolysis of cellulose
ترجمه فارسی عنوان
اثر لیگنین های باقی مانده موجود در کاه برنج یونیک قبل از درمان یون کلینیم بر روی هیدرولیز آنزیمی سلولز
کلمات کلیدی
واکنش زنجیره ای آنزیمی، مایعات یونی، زیست توده لیگنوسلولوزیک، جذب آنزیم غیر تولیدی، پیش درمان
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی


- RLs isolated from cholinium IL-pretreated straw had different structural features.
- Gly-RL had the highest carboxylic acid and the lowest phenolic hydroxyl contents.
- Gly-RL displayed the weakest non-productive enzyme adsorption.
- Negative effect of RLs stemmed from their inhibition to cellulase-mediated reactions.

To provide deep insights into cholinium ionic liquid (IL) pretreatment effect on the subsequent enzymatic hydrolysis, residual lignins (RLs) present in rice straw pretreated by cholinium glycinate ([Ch][Gly]), acetate ([Ch][OAc]), and glycolate ([Ch][Glc]) were isolated and characterized in this work. The effects of RLs on the enzymatic hydrolysis of cellulose and cellobiose, and their non-productive adsorption to β-glucosidase and cellulase were explored. Of the three RLs examined, RL isolated from [Ch][Gly]-pretreated straw (Gly-RL) had the highest carboxylic acid content (1.27 mmol/g) and the lowest phenolic hydroxyl content (0.72 mmol/g), resulting in the weakest non-productive enzyme adsorption. It might account well for the highest enzymatic digestibility of cellulose in the presence of Gly-RL. However, the strongest enzyme adsorption was found in the case of Glc-RL with the lowest carboxylic acid content (0.14 mmol/g) and the highest phenolic hydroxyl content (1.19 mmol/g). The enzymatic hydrolysis of cellulose was significantly affected by RLs. The negative effect of RLs on the cellulose hydrolysis mainly stemmed from their inhibition to cellulase-mediated reactions, instead of inhibition to β-glucosidase-catalyzed cellobiose hydrolysis.

Pretreatment with different cholinium ILs resulted in distinct lignin structural features, which had a significant effect on subsequent enzymatic hydrolysis.175

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Science - Volume 161, 6 April 2017, Pages 48-56
نویسندگان
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