کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6482089 | 364 | 2016 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dual stimulation of antigen presenting cells using carbon nanotube-based vaccine delivery system for cancer immunotherapy
ترجمه فارسی عنوان
تحریک دوگانه سلول های ارائه شده آنتی ژن با استفاده از سیستم تحویل واکسن مبتنی بر نانولوله کربنی برای ایمونوتراپی سرطان
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کلمات کلیدی
نانولوله های کربنی، سلولهای دندریتیک، نانومواد تحویل واکسن، واکسن های سرطانی،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Although antiâcancer immunoâbased combinatorial therapeutic approaches have shown promising results, efficient tumour eradication demands further intensification of antiâtumour immune response. With the emerging field of nanovaccinology, multiâwalled carbon nanotubes (MWNTs) have manifested prominent potentials as tumour antigen nanocarriers. Nevertheless, the utilization of MWNTs in coâdelivering antigen along with different types of immunoadjuvants to antigen presenting cells (APCs) has not been investigated yet. We hypothesized that harnessing MWNT for concurrent delivery of cytosineâphosphateâguanine oligodeoxynucleotide (CpG) and anti-CD40 Ig (αCD40), as immunoadjuvants, along with the model antigen ovalbumin (OVA) could potentiate immune response induced against OVAâexpressing tumour cells. We initially investigated the effective method to coâdeliver OVA and CpG using MWNT to the APC. Covalent conjugation of OVA and CpG prior to loading onto MWNTs markedly augmented the CpGâmediated adjuvanticity, as demonstrated by the significantly increased OVAâspecific T cell responses in vitro and in C57BL/6 mice. αCD40 was then included as a second immunoadjuvant to further intensify the immune response. Immune response elicited in vitro and in vivo by OVA, CpG and αCD40 was significantly potentiated by their coâincorporation onto the MWNTs. Furthermore, MWNT remarkably improved the ability of coâloaded OVA, CpG and αCD40 in inhibiting the growth of OVAâexpressing B16F10 melanoma cells in subcutaneous or lung pseudoâmetastatic tumour models. Therefore, this study suggests that the utilization of MWNTs for the coâdelivery of tumourâderived antigen, CpG and αCD40 could be a competent approach for efficient tumours eradication.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 104, October 2016, Pages 310-322
Journal: Biomaterials - Volume 104, October 2016, Pages 310-322
نویسندگان
Hatem A.F.M. Hassan, Lesley Smyth, Julie T.-W. Wang, Pedro M. Costa, Kulachelvy Ratnasothy, Sandra S. Diebold, Giovanna Lombardi, Khuloud T. Al-Jamal,