کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6482119 | 1318699 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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چکیده انگلیسی
Cherry virus A (CVA) is a member of the genus Capillovirus, in the family Betaflexiviridae. The infection rate of CVA was high in sweet cherry in China. We determined the complete nucleotide sequences of two isolates of CVA from Tai'an, Shandong Province, China using high fidelity PCR enzymes and specific primer pairs for amplifying long fragments in RT-PCR and RACE. The full-length sequences from isolates ChTA11 and ChTA12 are both 7 382 nucleotide (nt) long, excluding the poly(A) tail, encode two open reading frames (ORFs) and have similar genome organization to the two isolates in Gen-Bank. The complete nucleotide sequence of ChTA11 is 98.2 and 81.2% nt identity to the isolates from Germany and India in GenBank, respectively, and the ChTA12 isolate is 98.2 and 81.0% similar. Analysis of the nucleotide and amino acid sequences showed that the domain of unknown function (DUF1717) is more variable compared with other domains. This is the first report of the complete nucleotide sequences of CVA isolates infecting sweet cherry in China.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Integrative Agriculture - Volume 15, Issue 7, July 2016, Pages 1667-1671
Journal: Journal of Integrative Agriculture - Volume 15, Issue 7, July 2016, Pages 1667-1671
نویسندگان
Rui GAO, Shi-fang LI, Mei-guang LU,