کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6484482 1416095 2018 45 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Histone deacetylase inhibitor treated cell sheet from mouse tendon stem/progenitor cells promotes tendon repair
ترجمه فارسی عنوان
سلول های حاوی مهار کننده هیستون دیاستیلاز از سلول های ساقه / پروتئین تاندون موش، باعث بهبود ترمیم تاندون می شود
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Tendon stem/progenitor cells (TSPCs) have been identified as a rare population in tendons. In vitro propagation is indispensable to obtain sufficient quantities of TSPCs for therapies. However, culture-expanded TSPCs are prone to lose their phenotype, resulting in an inferior repaired capability. And little is known about the underlying mechanism. Here, we found that altered gene expression was associated with increased histone deacetylase (HDAC) activity and expression of HDAC subtypes. Therefore, we exposed ScxGFP mice-derived TSPCs to HDAC inhibitor (HDACi) trichostatin A (TSA) or valproic acid (VPA), and observed significant expansion of ScxGFP+ cells without altering phenotypic properties. TSA upregulated Scx expression by inhibiting HDAC1 and -3, and increasing the H3K27Ac level of Tgfb1 and -2 genome region. Additionally, cell sheets formed from TSA-pretreated mTSPCs retained the ability to accelerate tendon repair in vivo. Thus, our results uncovered an unrecognized role of HDACi in phenotypic and functional mTSPCs expansion to enhance their therapeutic potential.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 172, July 2018, Pages 66-82
نویسندگان
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