کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6485639 | 403 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Availability of extracellular matrix biopolymers and differentiation state of human mesenchymal stem cells determine tissue-like growth in vitro
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To explore the space-filling growth of adherent mesenchymal stem cells (MSC) into tissue-like structures in vitro, human bone marrow derived MSC were exposed to fibronectin-coated, millimeter-sized, triangular channels casted in poly(dimethyl siloxane) carriers. The results revealed that the three dimensional (3D) growth of MSC differs in dependence on differentiation status and availability of extracellular matrix (ECM) proteins: Massive 3D structure formation was observed for MSC under pro-osteogenic stimulation but not for undifferentiated MSC nor for MSC under pro-adipogenic stimulation; boosting cellular matrix secretion and addition of soluble ECM proteins caused extensive 3D tissue formation of undifferentiated MSC. The reported findings may contribute to bridge the gap between in vitro and in vivo analyses and guide the application of MSC in tissue replacement approaches.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 60, August 2015, Pages 121-129
Journal: Biomaterials - Volume 60, August 2015, Pages 121-129
نویسندگان
Manuela Herklotz, Marina C. Prewitz, Cécile M. Bidan, John W.C. Dunlop, Peter Fratzl, Carsten Werner,