کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6485700 | 413 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Variants of self-assembling peptide, KLD-12 that show both rapid fracture healing and antimicrobial properties
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBSCHOLMALDI-TOFHRBCsNBDMICSFMOCSUVs - SUV هاALP - آلکالن فسفاتازAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییegg phosphatidylcholine - تخم مرغ فسفاتیدیل کولینegg phosphatidylglycerol - تخم مرغ فسفاتیدیل گلیسرولminimum inhibitory concentrations - حداقل غلظت مهاریmatrix-assisted laser desorption ionization time-of-flight - زمان پرواز یونیزاسیون لیزر جذب ماتریسPhosphate buffered saline - فسفات بافر شورSelf-assembling peptide - پپتید خودمراقبتیhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراcholesterol - کلسترولsmall unilamellar vesicles - کیسه های کوچک کوچکHuman red blood cells - گلبول های قرمز انسان
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
KLD-12 (KLD) is a 12-residue self-assembling peptide that can adopt nano-structures and is known for its tissue-engineering properties. Our objective was to introduce antimicrobial attribute to KLD which would help in preventing secondary infection associated with external application of such tissue engineering materials. Considering the net charge of KLD-12, varying number of cationic arginine residues were added to its N-terminus. KLD variants showed appreciable bactericidal properties without any significant increase in cytotoxicity against tested mammalian cells. Further, these variants adopted β-sheet structures and self-assembled into nano-structures comparable to that of KLD. Interestingly, the KLD variants with two (KLD-2R) and three (KLD-3R) arginine residues added to its N-terminus showed significant osteogenic effect which was comparable or better than the original peptide as evident from the alkaline phosphatase activity assay, mineralized nodule formation and expression of different osteogenic genes. Particularly, application of KLD-2R in rats to the site of a drill-hole (0.8 mm diameter) that was created in the femur metaphysis displayed significantly higher bone regeneration compared to that of KLD. The results demonstrate a simple way to improve biological property of a self-assembling peptide with tissue engineering property.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 56, July 2015, Pages 92-103
Journal: Biomaterials - Volume 56, July 2015, Pages 92-103
نویسندگان
Jitendra K. Tripathi, Subhashis Pal, Bhanupriya Awasthi, Amit Kumar, Anshika Tandon, Kalyan Mitra, Naibedya Chattopadhyay, Jimut Kanti Ghosh,