کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6485755 414 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Conversion of mouse fibroblasts into cardiomyocyte-like cells using small molecule treatments
ترجمه فارسی عنوان
تبدیل فیبروبلاست های ماوس به سلول های مانند سلول های کرومیوسیت با استفاده از درمان های کوچک مولکولی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The possibility of controlling cell fates by overexpressing specific transcription factors has led to numerous studies in stem cell research. Small molecules can be used, instead of transcription factors, to induce the de-differentiation of somatic cells or to induce pluripotent cells (iPSCs). Here we reported that combinations of small molecules could convert mouse fibroblasts into cardiomyocyte-like cell without requiring transcription factor expression. Treatment with specific combinations of small molecules that are enhancer for iPSC induction converted mouse fibroblasts into spontaneously contracting, cardiac troponin T-positive, cardiomyocyte-like cells. We specifically identified five small molecules that can induce mouse fibroblasts to form these cardiomyocyte-like cells. These cells are similar to primary cardiomyocytes in terms of marker gene expression, epigenetic status of cardiac-specific genes, and subcellular structure. Our findings indicate that lineage conversion can be induced not only by transcription factors, but also by small molecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 54, June 2015, Pages 201-212
نویسندگان
, , , , , , , , , , , , , ,