کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6485800 | 415 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human three-dimensional engineered neural tissue reveals cellular and molecular events following cytomegalovirus infection
ترجمه فارسی عنوان
بافت عصبی مهندسی سه بعدی انسان، رویدادهای سلولی و مولکولی را به دنبال عفونت سیتومگالوویروس نشان می دهد
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کلمات کلیدی
مهندسی بافت عصبی، سلول عصبی، سلول بنیادی، عفونت،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Human cytomegalovirus (HCMV) is the most common cause of congenital infection of the central nervous system (CNS). To overcome the limited access to human neural tissue and stringent species specificity of HCMV, we used engineered neural tissues to: (i) provide a technical advance to mimick features of HCMV infection in a human neural fetal tissue in vitro and (ii) characterize the molecular and cellular phenomenon following HCMV infection in this tissue. Herein, we infected hESC-derived engineered neural tissues (ENTs) whose organization resembles fetal brain. Transcriptome analysis of ENTs demonstrated that HCMV infection displayed features of the infection with the expression of genes involved in lipid metabolism, growth and development, as well as stress and host-response in a time-dependent manner. Immunohistochemical analysis demonstrated that HCMV did not firstly infect neural tubes (i.e. radially organized, proliferating stem cell niches), but rather an adjacent side population of post-mitotic cells expressing nestin, doublecortin, Sox1, musashi and vimentin markers. Importantly, we observe the same tropism in naturally HCMV-infected fetal brain specimens. To the best of our knowledge this system represents the first human brain-like tissue able to provide a more physiologically model for studying HCMV infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 53, June 2015, Pages 296-308
Journal: Biomaterials - Volume 53, June 2015, Pages 296-308
نویسندگان
Ãrika Cosset, Yannick Martinez, Olivier Preynat-Seauve, Johannes-Alexander Lobrinus, Caroline Tapparel, Samuel Cordey, Hedi Peterson, Tom J. Petty, Marilena Colaianna, Vannary Tieng, Diderik Tirefort, Andras Dinnyes, Michel Dubois-Dauphin,