کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6486509 440 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Prediction of inflammatory responses induced by biomaterials in contact with human blood using protein fingerprint from plasma
ترجمه فارسی عنوان
پیش بینی پاسخ های التهابی ناشی از مواد بیولوژیکی در تماس با خون انسان با استفاده از اثر انگشت پانل از پلاسما
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Inappropriate complement activation is often responsible for incompatibility reactions that occur when biomaterials are used. Complement activation is therefore a criterion included in legislation regarding biomaterials testing. However, no consensus is yet available regarding appropriate complement-activation-related test parameters. We examined protein adsorption in plasma and complement activation/cytokine release in whole blood incubated with well-characterized polymers. Strong correlations were found between the ratio of C4 to its inhibitor C4BP and generation of 10 (mainly pro-inflammatory) cytokines, including IL-17, IFN-γ, and IL-6. The levels of complement activation products correlated weakly (C3a) or not at all (C5a, sC5b-9), confirming their poor predictive values. We have demonstrated a direct correlation between downstream biological effects and the proteins initially adhering to an artificial surface after contact with blood. Consequently, we propose the C4/C4BP ratio as a robust, predictor of biocompatibility with superior specificity and sensitivity over the current gold standard.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 36, January 2015, Pages 55-65
نویسندگان
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