کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6588732 1423242 2018 25 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Construction of thermo-tolerant yeast based on an artificial protein quality control system (APQC) to improve the production of bio-ethanol
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Construction of thermo-tolerant yeast based on an artificial protein quality control system (APQC) to improve the production of bio-ethanol
چکیده انگلیسی
A major challenge for the production of bio-ethanol is to develop yeast that can sustain growth under high temperature. Here, we show the application of an artificial protein quality control (APQC) system to an industrial Saccharomyces cerevisiae strain for improving thermo-tolerance, a key trait for a bio-ethanol fermentation program. All engineered strains exhibited a significantly higher growth rate and cell viability at 37 °C, which conferred increased thermo-tolerance and more efficient glucose conversion to ethanol. Furthermore, the analysis of ubiquitination and the enzyme activities of pyruvate kinase (PK) and malate dehydrogenase (MDH) indicated strengthened protein quality, implying that proteostasis had been effectively improved at higher temperature. Industrial material fermentation results from yeast-RSP5-UBC4 (combined expression of ubiquitin conjugated enzyme E2 and ubiquitin ligase E3) showed a 2.4% increase in ethanol production. All these findings prove that APQC is suitable for improving yeast resistance to high temperature during industrial bio-ethanol production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Science - Volume 177, 23 February 2018, Pages 410-416
نویسندگان
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