کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6589913 | 456851 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Engineering batch and pulse refolding with transition of aggregation kinetics: An investigation using green fluorescent protein (GFP)
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
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چکیده انگلیسی
Pulse refolding is a strategy to overcome concentration dependent aggregation, assuming that aggregation is significantly suppressed under diluted conditions. When a typical 2nd or higher order aggregation kinetics is assumed, kinetics over predicted yields at low refolding concentrations. Using GFP as our model protein, we found a transition in aggregation kinetics from 2nd to 1st order when intermediates deplete from 100 to 60 µg/ml. Taking this transition into account, the model can better predict refolding yields in batch and pulse refolding strategies. This model is suited for the design of refolding processes since this deviation from 2nd or higher order aggregation was also previously observed in other proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Science - Volume 131, 28 July 2015, Pages 91-100
Journal: Chemical Engineering Science - Volume 131, 28 July 2015, Pages 91-100
نویسندگان
Siqi Pan, Nora Odabas, Bernhard Sissolak, Moritz Imendörffer, Monika Zelger, Alois Jungbauer, Rainer Hahn,