کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6599171 | 1423884 | 2018 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Facile synthesis of green-light and large Stokes-shift emitting coumarins for bioconjugation
ترجمه فارسی عنوان
سنتز لایه ای از کومارین های سبز نور و بزرگ استوکس تغییر جهت بیوک کنجوج شدن
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
چکیده انگلیسی
Bright and hydrosoluble fluorescent dyes with large Stokes shifts are widely used as molecular probes and light-emitting markers in chemistry and biology. In this report, one-step synthesis of a bright green-emitting coumarin (Φâ¯=â¯0.52, λemâ¯=â¯505â¯nm) was achieved from available reagents under neat conditions. The novel dye 1 showed very large Stokes shift (Îλâ¯=â¯138â¯nm) due to strong intramolecular charge transfer (ICT) effect, which was confirmed by density functional theory (DFT) calculations. Dye 1 was hydrosoluble and displayed strong emission at a wide range of pH from 3 to 8. Importantly, the secondary amine in 1 made it extremely convenient for futher bioconjugation. Representative derivatives were showcased for various successful applications of fluorogenic enzyme assay, biolabelling and mitochondrial staining.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dyes and Pigments - Volume 151, April 2018, Pages 303-309
Journal: Dyes and Pigments - Volume 151, April 2018, Pages 303-309
نویسندگان
Jiayuan Li, Changyu Zhang, Shuai Wu, Xin Wen, Zhen Xi, Long Yi,