کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6601350 459332 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Paper-based electrochemiluminescence biosensor for cancer cell detection
ترجمه فارسی عنوان
بیوسنسور الکتروکوهیمیلومینسانس کاغذی برای تشخیص سلول های سرطانی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی
Here a novel paper-based electrochemiluminescence (ECL) device was developed for the detection of HL-60 cancer cells. In this approach, porous filter paper as a thin-layer electrochemical cell connected to a sheet of ITO working electrode. Au nanoparticles (Au NPs) and graphene were coated on the surface of ITO to immobilize capture HS-DNA. Then, the aptamer of HL-60 cancer cells, which is labeled with Ru(bpy)32 +-conjugated silica nanoparticles (RuSi@Ru(bpy)32 +) is hybridized with the capture DNA to form bioconjugates. In the presence of HL-60 cells, the aptamer would conjugate with glycoprotein at cell surface and RuSi@Ru(bpy)32 + NP-aptamer would be released. After the dissociation of RuSi@Ru(bpy)32 + NP-aptamer from the surface of ITO, ECL signal would decline. This simple, portable and disposable testing platform could detect HL-60 cancer cells down to 56 cells/mL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochemistry Communications - Volume 49, December 2014, Pages 88-92
نویسندگان
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