Protein reducing agents dithiothreitol and tris(2-carboxyethyl)phosphine anodic oxidation

► Clarify the redox mechanism of two protein reducing agents dithiothreitol (DTT) or tris(2-carboxyethyl)phosphine (TCEP). ► pH-dependent oxidation process investigated at a glassy carbon electrode. ► Determination of diffusion coefficients of DTT and TCEP.