Purification of algal anti-tyrosinase zeaxanthin from Nannochloropsis oculata using supercritical anti-solvent precipitation

▶ Nannochloropsis oculata with high content of algal zeaxanthin was cultivated by sea water. ▶ The extract of microalgae was fractionated by elution chromatography and was then purified by supercritical anti-solvent. ▶ Sub-micron zeaxanthin-rich particulates presented anti-tyrosinase ability.