Effect of polyols on the native structure of α-chymotrypsin: A comparable study

► We have studied stability of α-chymotrypsin in polyols. ► We have performed DSC, CD and fluorescence spectroscopy. ► Our ΔGu of CT in polyol increase as polyol concentration increases. ► All polyols acted as enhancers for CT stability. ► Our results show that trehalose is strong stabilizer.