کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
674667 1459572 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Studies of irreversible heat denaturation of green fluorescent protein by differential scanning microcalorimetry
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی جریان سیال و فرایندهای انتقال
پیش نمایش صفحه اول مقاله
Studies of irreversible heat denaturation of green fluorescent protein by differential scanning microcalorimetry
چکیده انگلیسی

Heat denaturation of green fluorescent protein (the GFP-cycle3 mutant) was studied by the method of differential scanning microcalorimetry. Activation energy values for two stages of GFP unfolding were calculated from the calorimetric data using the model of irreversible denaturation. Dependences of activation energy and denaturation enthalpy on the temperature of the maxima of corresponding stages of denaturation were obtained, which allow estimating the corresponding increments of heat capacity. Based on the known correlations of the structure and energy parameters, it was concluded that the first transition state is close to the native state, whereas the second transition state is close to the denatured state, judging by the exposure of hydrophobic groups to the solvent.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Thermochimica Acta - Volume 512, Issues 1–2, 10 January 2011, Pages 71–75
نویسندگان
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