Physicochemical characterization of alkali pretreated sugarcane tops and optimization of enzymatic saccharification using response surface methodology

Alkali pretreatment of sugarcane tops was carried out with 3% NaOH for 60 min at 121 °C in a laboratory autoclave. The effect of solid loading, enzyme loading, incubation time and surfactant concentration on enzymatic saccharification was studied using a response surface method according to Box-Behnken design. Under optimized conditions 77.5% sugar was recovered from the pretreated biomass. This yield was seven times higher than that obtained with untreated sugarcane tops. A substantial amount of lignin (90%) was removed by this pretreatment method. Physicochemical characterization of native and alkali pretreated sugarcane tops were carried out by XRD, FTIR and SEM and the changes in the chemical composition were also monitored. The X-ray diffraction profile showed that the degree of crystallinity was higher for alkali pretreated biomass than that for native.