کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6858 522 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A gold nanoparticle platform for the delivery of functional microRNAs into cancer cells
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
A gold nanoparticle platform for the delivery of functional microRNAs into cancer cells
چکیده انگلیسی

Lack of affordable technologies for delivering microRNAs and siRNAs into cells on a large scale has hindered our efforts to rapidly parse through hundreds of dysregulated genes/microRNAs in order to identify drivers of complex diseases. The instability and polyanionic nature of naked microRNAs impede efficient cellular uptake and reduce half-life. Viral delivery requires cloning, microRNA mimics/inhibitors require costly modifications, and both require toxic lipofection or electroporation. To address these challenges, we developed a robust method for delivering unmodified microRNAs into cells on cysteamine-functionalized gold nanoparticles (AuNPs). We validated our method in two different tumor models and found that the best formulation of miR1-AuNP10-S-PEG0.5 had the highest payload (10–20 fold higher than lipofection), lowest toxicity (98% of cell viability following treatment), efficient uptake (96% of cells took it), fastest endosomal escape and increased half-lives (at least 5 days) impacting cell proliferation and patterns of target gene expression.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 3, January 2013, Pages 807–816
نویسندگان
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