کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7043804 | 1456920 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An efficient approach for the enzyme-enhanced extraction of camptothecin and 10-hydroxycamptothecin from the samara of Camptotheca acuminata using an ionic liquid solution
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
An efficient approach for enzyme-enhanced extraction using an ionic liquid solution (ILEEE) of the major alkaloidal components camptothecin (CPT) and 10-hydroxycamptothecin (HCPT) from the samara of Camptotheca acuminata is presented. The enzyme pretreatment in an aqueous ionic liquid (IL) media was used for the enhancement of extraction yield. The activity of hemicellulase was investigated in various ILs with imidazolium-based cations ([C2MIM]+, [C4MIM]+, [C6MIM]+, [C8MIM]+, [C10MIM]+) and anions (Brâ, Clâ, Acâ, HSO4â, NO3â). The optimal ionic liquid for the hemicellulase extraction process was found to be a 0.75â¯M [C8MIM]Br aqueous solution. Single factor experiments and Box-Behnken design found the optimal operational parameters for ILEEE to be an incubation temperature of 40â¯Â°C, incubation time of 12â¯h, extraction phase of pH 4, liquid-solid ratio of 30â¯mL/g, 0.75â¯M [C8MIM]Br and a hemicellulase concentration of 0.441â¯mg/mL. Under these optimized extraction conditions, the total yield of CPT and HCPT was 1043â¯Â±â¯51.2â¯Î¼g/g. SEM images of the samples revealed that hemicellulases hydrolyze the cell wall, leading to structure breakdown, which enabled [C8MIM]Br to have a high dissolving power for the targeted components. These factors are the main causes for the increasing in the total yield of the CPT and HCPT when using the ILEEE extraction method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 200, 12 July 2018, Pages 102-111
Journal: Separation and Purification Technology - Volume 200, 12 July 2018, Pages 102-111
نویسندگان
Guoqiang Cui, Xuebing Yang, Tingting Liu, Zaizhi Liu, Lei Yang,