کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7044407 | 1456997 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Confocal laser scanning microscopy as a tool to validate the efficiency of membrane cleaning procedures to remove biofilms
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تصفیه و جداسازی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The efficiency of six different cleaning protocols was studied and quantified by measuring ATP and carbohydrate (CH) concentrations. The detailed characterization of changes in the biofilm structure due to the application of various cleaning agents was performed by means of confocal laser scanning microscopy (CLSM). Biofilm samples were cultivated over a period of 28Â days under well-defined conditions in space and time to mimic seriously biofouled membrane surfaces which were tested under reproducible conditions. Quantitative structural parameters were calculated from CLSM image stacks. Imaging data revealed visually and quantitatively differences at the microscale in the behavior of cleaning procedures applied. Moreover, these data showed correlations with quantitative biomass parameters ATP and CH at the macroscale. Hence, this study supports the use of CLSM as a research tool to understand in detail the effects of selected cleaning agents on biofilm structure and presents supportive data for the efficacy of certain cleaning protocols.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Separation and Purification Technology - Volume 122, 10 February 2014, Pages 402-411
Journal: Separation and Purification Technology - Volume 122, 10 February 2014, Pages 402-411
نویسندگان
S. West, H. Horn, W.A.M. Hijnen, C. Castillo, M. Wagner,