کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7064437 | 1459825 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Growth under field conditions affects lignin content and productivity in transgenic Populus trichocarpa with altered lignin biosynthesis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
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چکیده انگلیسی
This study evaluated the potential of transgenic Populus trichocarpa with antisense 4CL for reduced total lignin and sense Cald5H for increased S/G ratio in a short rotation woody cropping (SRWC) system for bioethanol production in the Southeast USA. Trees produced from tissue-culture were planted in the Coastal Plain, Piedmont, and Mountain regions of North Carolina, USA. Trees were observed for growth differences and biomass recorded for two coppices. Insoluble lignin and S/G ratio were determined by molecular beam mass spectroscopy after the second coppice. Survival, growth form, and biomass were very consistent within construct lines. Higher total lignin content and S/G ratio were positively correlated with total aboveground biomass. The low-lignin phenotype was not completely maintained in the field, with total lignin content increasing on average more than 30.0% at all sites by the second coppice The capacity to upregulate lignin in the event of environmental stress may have helped some low-lignin lines to survive. More research focused on promising construct lines in appropriate environmental conditions is needed to clarify if a significant reduction in lignin can be achieved on a plantation scale, and whether that reduction will translate into increased efficiency of enzymatic hydrolysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomass and Bioenergy - Volume 68, September 2014, Pages 228-239
Journal: Biomass and Bioenergy - Volume 68, September 2014, Pages 228-239
نویسندگان
Anna T. Stout, Aletta A. Davis, Jean-Christophe Domec, Chenmin Yang, Rui Shi, John S. King,