کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7064540 | 1459826 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Influences of carbon sources on the biomass, production and compositions of exopolysaccharides from Paecilomyces hepiali HN1
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Influences of carbon sources on the biomass, production and compositions of exopolysaccharides from Paecilomyces hepiali HN1 Influences of carbon sources on the biomass, production and compositions of exopolysaccharides from Paecilomyces hepiali HN1](/preview/png/7064540.png)
چکیده انگلیسی
The parasitic fungus, Paecilomyces hepiali, is used to produce Cordyceps materials as succedaneum of natural Cordyceps sinensis in China. The purpose of this research was to investigate the effects of glucose, mannose, sucrose, lactose as solo carbon source and sucrose + lactose or mannose + sucrose as synthetic carbon source on the growth of mycelium and production, chemical composition, molecular weight distribution and monosaccharide composition of exopolysaccharides from P. hepiali HN1 (PHEPS). The maximum mycelium biomass of 12.16 kg mâ3 and PHEPS yield of 4.57 kg mâ3 were achieved from the culture with sucrose (50 kg mâ3) as carbon source. The resulting PHEPS was characterized by analyses of chemical composition, size-exclusion chromatography and high performance liquid chromatography with 1-phenyl-3-methyl-5-pyrazolone pre-column derivatization. It was found that the chemical compositions and monosaccharide ratios in PHEPS were significantly affected by the carbon sources used. Glucose or mannose as carbon source enhanced the biosynthesis of PHEPS with higher-molecular weight (>1000 kD), but solo carbon source of lactose or synthetic carbon source of mannose + lactose did not increase the ratio of galactose in PHEPS. The metabolism kinetics of carbon sources demonstrated the correlation between PHEPS synthesis and the utilization of carbon sources. These findings will be useful for further works on the production, structure and function of PHEPS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomass and Bioenergy - Volume 67, August 2014, Pages 260-269
Journal: Biomass and Bioenergy - Volume 67, August 2014, Pages 260-269
نویسندگان
Zhongwei Wu, Zijiang Yang, Dan Gan, Jialong Fan, Zhuqing Dai, Xiaoqing Wang, Bing Hu, Hong Ye, Muhammad Abid, Xiaoxiong Zeng,