Separation of overlapped chromosomes and pairing of similar chromosomes for karyotyping analysis

Chromosome analysis gives a better solution to prevent most of the genetic disorders. Many methods for chromosome analysis were proposed and most of them require human intervention at some point. The main initial problem is that the chromosomes are non-rigid in nature and they can be overlapped or touching with other chromosome images during cell division. This work mainly concentrate on (i) separation of overlapped chromosome images, (ii) homologue chromosome identification by centromere position. The initial step is to segment and separate the overlapped chromosome images. An automatic geometry separation algorithm is developed which helps in separating the overlapped chromosome images. The main step is to identify the cut points on the image. From the obtained points, a proper cut line is obtained over the overlapped region. The cut line indicates the separation zone. Hypothesis verification is done for obtaining the proper disentangled chromosome images. The accuracy of the algorithm is 94%.