کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7138640 | 1462014 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrochemical genosensor based on carboxylated graphene for detection of water-borne pathogen
ترجمه فارسی عنوان
ژنوسنسور الکتروشیمیایی بر اساس گرافن کربوکسیل شده برای تشخیص پاتوژن منتقل شده از آب
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
This work reports the application of newly synthesized carboxylated graphene nanoflakes (Cx-Gnfs). The Cx-Gnfs were synthesized by wet chemical method in sulfuric acid/nitric acid mixture and was further electrophoretically deposited on indium tin oxide (ITO) coated glass substrates using Mg2+ ions which provides an overall charge to the materials for deposition onto the anode. The materials were characterized using SEM, TEM, contact angle, UV-vis spectroscopy, FT-IR, XRD and electrochemically characterized by cyclic voltammetry, chronocoulometry and electrochemical impedance spectroscopy. The sensitive quantitative determination of nucleic acid Escherichia coli O157: H7 (E. coli) has been achieved using Cx-Gnfs and r-GO as the sensing layer using electrochemical impedance spectroscopy. The electrochemical results reveal that the Cx-Gnfs based genosensor exhibits a linear response to complementary DNA (10â6 M to 10â17 M) with a detection limit of 1âÃâ10â17 M while the rGO based genosensor shows a detection limit of 1âÃâ10â15 M. Under optimal conditions, this Cx-Gnfs based genosensor was found to retain about 85% of its initial activity after being used for 6 times.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 275, 1 December 2018, Pages 312-321
Journal: Sensors and Actuators B: Chemical - Volume 275, 1 December 2018, Pages 312-321
نویسندگان
Nandita Jaiswal, Chandra Mouli Pandey, Amrita Soni, Ida Tiwari, Martin Rosillo-Lopez, Christoph G. Salzmann, Bansi Dhar Malhotra, Gajjala Sumana,