کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7138676 1462015 2018 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid detection of heparin by gold nanorods and near-infrared fluorophore ensemble based platform via nanometal surface energy transfer
ترجمه فارسی عنوان
تشخیص سریع هپارین توسط نانوذرات طلا و پلت فرم مبتنی بر مجموعه فلوئوروفور نزدیک مادون قرمز از طریق انتقال انرژی نانوذرات
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
As a mucopolysaccharide sulfate anticoagulant drug, heparin plays important roles in clinic. In this work, heparin was analyzed based on the nanometal surface energy transfer (NSET) between gold nanorods (AuNRs) and tetrakis (4-sulfophenyl) porphyrin (TPPS4) fluorescent molecules. The results showed that AuNRs could bind TPPS4 by electrostatic interaction and coordination to quench its fluorescence. While heparin was able to bind AuNRs with a stronger electrostatic interaction to compel TPPS4 to leave AuNRs surface, leading to the fluorescence recovery of TPPS4, which was proportional to the concentration of heparin in a range of 0.05∼2 μg/mL with the detection limit as low as 6.7 ng/mL (3σ). Based on this, a NSET platform for the analysis of trace heparin sodium was proposed with fast response, high sensitivity and excellent specificity, which was successfully applied to the detection of heparin in injection with the satisfactory result. Importantly, the NSET mechanism was fully discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 274, 20 November 2018, Pages 318-323
نویسندگان
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