کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7139777 1462024 2018 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Impedimetric paper-based biosensor for the detection of bacterial contamination in water
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Impedimetric paper-based biosensor for the detection of bacterial contamination in water
چکیده انگلیسی
According to the World Health Organisation, worldwide waterborne diseases are responsible for nearly two million human deaths annually. Rapid and at-site screen of pathogenic microorganisms in drinking water can help to markedly reduce this number. Here we report an innovative, simple and low-cost, paper-based probe for detection of bacteria in water, fabricated by screen printing carbon electrodes onto hydrophobic paper. Electrochemical characterization of the printed electrodes confirmed fast-electron transfer, with an estimated electroactive surface area of 0.25 cm2. The electrode surface was functionalised with carboxyl groups, prior to covalent immobilization of the lectin Concanavalin A (Con A), used as the biorecognition element. The system was then tested as an impedimetric sensor for bacteria in water. A linear increase in the probe charge transfer resistance was observed for bacterial concentrations ranging from 103 to 106 CFU mL−1, with an estimated lower detection limit of 1.9 × 103 CFU mL−1. Considering its remarkable simplicity, cost-effectiveness and biodegradability, the sensor here reported could be an attractive solution for portable testing kits that address the challenges of traditional time-consuming and expensive lab-based analyses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 265, 15 July 2018, Pages 50-58
نویسندگان
, , , ,