کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7139924 | 1462024 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Colorimetric detection of Listeria monocytogenes using one-pot biosynthesized flower-shaped gold nanoparticles
ترجمه فارسی عنوان
تشخیص رنگ سنجی لیستریا مونو سیتوژنز با استفاده از نانوذرات طلای گل طلایی با غلظت سفید
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The present study described a novel fast, simple and green one-pot synthesis approach of flower-shaped gold nanoparticles (flower-shaped AuNPs) by using antioxidant plant extracts. The AuNPs synthesized by Alchemilla mollis extract showed maximum absorbance at 610â¯nm and were polydispersed flower-shaped with average size (±SD) of 68.7â¯Â±â¯2.8â¯nm. We have explored a colorimetric sensing assay for rapid detecting Listeria monocytogenes by using single-stranded DNA (ssDNA) conjugated flower-shaped AuNPs. ssDNA were used as probe for DNA detection and the synthesized flower-shaped AuNPs were used as indicators. The limit of detection for hlyA gene and genomic DNA of Listeria monocytogenes were 48.4â¯ng and 100.4â¯ng, respectively. The proposed assay is fast and specific for Listeria monocytogenes detection, with the improved color change and stability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 265, 15 July 2018, Pages 285-292
Journal: Sensors and Actuators B: Chemical - Volume 265, 15 July 2018, Pages 285-292
نویسندگان
Juan Du, Hina Singh, Wei-jie Dong, Yan-hong Bai, Tae-Hoo Yi,