کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7142215 1462052 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Probing putative carcinogenic potential of processed and unprocessed meat using bioluminescent bacterial bioreporters
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Probing putative carcinogenic potential of processed and unprocessed meat using bioluminescent bacterial bioreporters
چکیده انگلیسی
Consumption of meat is increasing worldwide and to prolong both it's shelf-life and safety companies add various chemicals, some of which are under evaluation to assess their responsible cancer induction. The International Agency of research on Cancer classified red meat and processed meat as probably carcinogenic (Group 2A) and carcinogenic (Group 1) respectively for humans. The aim of this study was to propose a biological sensor able to detect DNA mutations that could lead to cancer induction in beef, and chicken as well as processed meats. Genetically engineered E. coli DPD2794 strain is able to produce bioluminescence in the presence of damages in the DNA molecule, allowing for the screening and discrimination between different meats in terms of potential carcinogenic induction. Unprocessed beef and chicken induced low amounts of bioluminescence, whereas processed beef and chicken induced values above the cut-off limit established. These values where low in comparison to the bioluminescence produced by a strong activator used as our positive reference, leading us to the conclusion that processed meat a low carcinogenic effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 239, February 2017, Pages 113-119
نویسندگان
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