Enhanced electrochemical biosensing of Buprenorphine opioid drug by highly stabilized magnetic nanocrystals

Herein, we report the synthesis of a spherically stabilized monodisperse magnetic nanocrystal for drug sensing. An optimized ratio of carbon paste material with the magnetic nanocrystal was used for electrochemical sensing of buprenorphine. The buprenorphine cathodic peak current Ip at CS-MNs/CP electrode was 10 fold higher than that of unmodified CP electrode. CS-MNCs/CP electrode is applicable to determine buprenorphine in the linear range of 0.02-68.0 μM with a limit of detection (LOD) of 4.3 nM. Fast response time, high sensitivity and ease of preparation method without using any specific electron transfer mediator or specific reagent are the advantages of the sensor. The modified electrode was applied to the determination of buprenorphine in human biological fluid at micromolar level without any pretreatment or separation step.