کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7142547 | 1462053 | 2017 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Highly selective detection of disulfenylated proteins through a dimedone-based fluorescent probe and application in cells
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A novel fluorescent probe, 2, 4-cyclohexanedione-1-propyl-pyrene (TAR), has been synthesized to research thioredoxin (Trx) disulfenylation and visualize the activity of Trx in cells. The turn-on fluorescence change was induced by combining the vicinal dithiol proteins with the spatially sensitive fluorescent signal of the pyrene excimer which emitted at 480Â nm. Trx activity can be detected at nanomolar concentrations. Trx preference of TAR in vitro and in vivo was proved by fluorometric and confocal microscopic experiments. In addition, it could realize dynamic tracking of protein disulfenylation in situ with minimal disturbance to disulfenylated proteins and less perturbation with cellular function. We herein report the first turn-on fluorescent sensor for the selective detection of proximally disulfenylated protein sites, suitable for application in both aqueous solutions and in cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 238, January 2017, Pages 257-263
Journal: Sensors and Actuators B: Chemical - Volume 238, January 2017, Pages 257-263
نویسندگان
Ting-Xiang Liu, Li Zhang, Ru-Ping Liang, Jian-Ding Qiu,