کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7142611 | 1462053 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A bicyclo-hairpin probe mediated strand displacement amplification strategy for label-free and sensitive detection of bleomycin
ترجمه فارسی عنوان
یک پروب دوچرخه موی زدنده استراتژی تقویت جابجایی رشته ها را برای تشخیص بیولوژیک و حساسیت بلومویایسین
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
پروب دوچرخهسواری تقویت جابجایی رشته، بدون برچسب بلومایسین،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Monitoring of bleomycin concentration is critical in clinical therapy. Here, a bicyclo-hairpin probe mediated strand displacement amplification strategy was developed for bleomycin detection. Firstly, bleomycin cleaved bicyclo-hairpin probe to release a trigger sequence. Then the trigger sequence initiated strand displacement amplification reaction to produce numerous G-quadruplex forming sequences. Finally, G-quadruplexes bound with N-methyl mesoporphyrin IX molecules and an enhanced fluorescent signal could be detected. In this strategy, a bicyclo-hairpin probe was ingeniously designed for bleomycin detection, which could silence the trigger sequence to reduce non-specific hybridization of detection method. In addition, based on bleomycin-induced DNA scission, a label-free fluorescent sensor for bleomycin detection was established. Our strategy could determine bleomycin sensitively with a detection limit of 0.34Â nM. Fluorescent response of detection system toward four different anti-tumor drugs proved that this strategy had admirable selectivity. Satisfactory recoveries in human serum samples revealed that our method had a great potential in real sample assay.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 238, January 2017, Pages 318-324
Journal: Sensors and Actuators B: Chemical - Volume 238, January 2017, Pages 318-324
نویسندگان
Huijuan Wang, Wei Jiang, Wei Li, Lei Wang,