کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7143657 1462059 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Integration of ring nanoelectrodes into microwells for the bioelectrochemical analysis in sub-picolitre volumes
ترجمه فارسی عنوان
یکپارچه سازی نانو الکترودهای حلقه ای به میکرووئل ها برای تجزیه و تحلیل بیو الکتروشیمیایی در حجم های زیر پیکولیتور
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this work, we report the fabrication and the electrochemical characterization of recessed disk microelectrodes (DME) and ring nanoelectrodes (RNE) integrated in microwell arrays. Such configuration has all advantages of microelectrodes arrays but is more suitable for electrochemical measurement in sub-picolitre volumes (∼0.3 pL). The technological process based on the reactive ion etching of a SiO2/Ti/Pt/Ti/SiO2 stack is optimized in order to integrate RNE arrays on transparent glass substrate. Multiphysic simulations and electrochemical characterizations are conducted in order to study and improve the amperometric behaviour of recessed ring nanoelectrodes according to their geometry. A good fit is shown between experimental, theoretical and simulation results, allowing full understanding of the electrochemical detection properties of RNE-based microwell arrays. Then, a “generation − collection mode” chronoamperometric approach is proposed to evaluate experimentally the collection ratio of RNE arrays and compare it with simulation results. Finally, first electrochemical characterizations in sub-picolitre volumes are conducted with anti-oxidant species. All these results demonstrate that recessed ring nanoelectrode arrays are fitted to the detection of bio-electrochemical species at the microscale and, consequently to single mitochondrion or single sub-cellular organelle analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 232, September 2016, Pages 345-356
نویسندگان
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