کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7144018 | 1462058 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzyme-free detection of DNA based on hybridization chain reaction amplification and fluorescence resonance energy transfer
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A versatile enzyme-free deoxyribonucleic acid (DNA) detection method based on hybridization chain reaction (HCR) and fluorescence resonance energy transfer (FRET) was developed. Hairpin H1 and H2 are labelled with carboxyfluorescein (FAM) as the donor and tetramethylrhodamine (TAMRA) as the acceptor, respectively. In the presence of target DNA, long nicked dsDNA nanowires are self-assembled through HCR process. The donor and the acceptor are brought in close proximity, resulting in a FRET process between them. The ratio of fluorescent intensities of the acceptor and donor can be used to quantitatively detect the target DNA with a limit of detection 0.7 nmol Lâ1. This proposed method was applied to detect DNA in biologic samples with satisfactory results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 233, 5 October 2016, Pages 691-696
Journal: Sensors and Actuators B: Chemical - Volume 233, 5 October 2016, Pages 691-696
نویسندگان
Ying Chen, Ling Chen, Yidian Ou, Liangqia Guo, Fengfu Fu,