Detection of Escherichia coli with a label-free impedimetric biosensor based on lectin functionalized mixed self-assembled monolayer

- An lectin-based EIS biosensor for the determination of Escherichia coli (E. coli) was developed based on lectin functionalized mixed self-assembled monolayer on gold electrode as sensing interface.
- The binary interface could reduce the nonspecific adsorption and improve the sensitivity of the sensor with the detection limit of 75 cells/mL for E. coli.
- The EIS biosensor could discriminate gram-negative E. coli from gram-positive bacteria.
- This strategy offers advantages of simple fabrication of lectin interface with high sensitivity and rapid determination.