The novel pillar[5]arene derivative for recyclable electrochemical sensing platform of homogeneous DNA hybridization

In this work, a recyclable electrochemical sensing platform to detect breast cancer susceptibility gene (BRCA) was constructed by pillararenes based host-guest recognition and homogeneous DNA hybridization. BRCA target DNA (T-DNA) formed sandwich-type DNA via homogeneous hybridization with methylene blue labeled signal DNA and alkylamino modified capture DNA, which could form complexes with pillararenes. Such sandwich-type DNA was captured by a novel trithiocarbonate modified pillar[5]arene (P5A-CTA) which was immobilized on the Au electrode. With the help of enzyme amplification, the electrochemical detection signal of target DNA in sensing platform was apparently amplified. This sensing platform exhibited wide linear range and excellent specificity, and was particularly recyclable after simple washing with hot acetonitrile due to the reversible host-guest complexation between P5A-CTA and alkylamino modified DNA.